The metabolism of nitrogen, digestibility coefficient and biological value of the proteins and net protein utilization in children of a protein food based on a blend of groundnut Bengalgram and sesame flours fortified with DL-methionine and L-lysine
Guttikar, M.N.; Panemangalore, M.; Doraiswamy, T.R.; Rao, M.N.; Rajalakshmi, D.; Swaminathan, M.
Journal of Nutrition and Dietetics 2: 113-118
Eight boys aged 9 to 12 years, belonging to lowincome groups of the Indian population and resident in a boarding home in Mysore city, were fed during four 10-day periods on a diet of low protein content, a similar diet supplemented daily with 88 g of a mixture of groundnut, Bengal gram (Cicer arietinum) and sesame flour in proportions of 4: 4: 2, or that mixture with DL-methionine to raise the total Samino acid intake to 4.3 g per 16 g N and L-lysine to give a total lysine intake of 6.3 g per 16 g N, or 100 g skimmed milk powder reconstituted with 600 ml water. The daily protein intakes on the 4 diets were 2.9, 36.5, 36.8 and 38.2 g and energy intakes were 1744, 1752, 1755 and 1748 kcal. Vitamin and mineral supplements were given where necessary so that all diets satisfied recommended requirements for children. Urinary and faecal N excretions during the final 4 days of each period and the essential amino acid contents of the diets were estimated. Protein scores by the FAO reference pattern and that of Swaminathan (Title 655, Vol. 35) were 73 and 51 for the diet with the mixture of groundnut, Bengal gram and sesame, 79 and 56 when lysine and methionine were added and 81 and 70 for the diet containing skimmed milk powder. The boys were in negative N balance on the diet free of protein but achieved mean positive balances of 1.36, 1.95 and 2.16 mg per kg bodyweight, respectively, on the diets supplemented with groundnut, Bengal gram and sesame, with methionine and lysine or with milk.