Biological properties of avirulent strains of Clostridium chauvoei.
Trudy Vsesoyuznogo Instituta Eksperimental' noi Veterinarii 39: 178-183
Eight Cl. chauvoei strains obtained from old culture collections were characterized in terms of their pathogenicity and immunogenicity for guinea-pigs. When guinea-pigs weighing 350-450 g were injected s/c with a 24-48 hour culture of each strain in doses ranging from 0.8 to 8 ml, four strains at 0.8 ml killed guinea-pigs in 18-72 hours, while the other four were avirulent even at 8 ml. None of the avirulent strains became pathogenic for guinea-pigs after ten guinea-pig passages.