Inactivation of influenza virus with binary ethylenimine

Wei, X.L.; Liu, J.; Gao, J.X.

Chinese Journal of Biologicals 21(5): 395-397


Objective: To observe the inactivation effect of binary ethylenimine (BEI) on influenza virus. Methods: Inactivate influenza virus with formaldehyde and BEI respectively, determine the inactivation effect by HI test and optimize the time for inactivation. Determine the antigenicity of inactivated virus by immunization of mice. Analyze the integrity of genome of inactivated virus by RT-PCR. Lyze influenza virus with Triton X-100 and inactivate with formaldehyde and BEI respectively.