The Virulence of Str. agalactiae from Clinically Normal Udders
Klimmer, M.; Haupt, H.
Berliner und Münchener Tierärztliche Wochenschrift 1938: 289-291
The method of testing the virulence of the strains was by attempts to establish infection by fastening small waterproof bags containing culture (saccharose broth containing chalk) to the udder and leaving them in situ by night tor about a week. The strains were from cows which had shown a long standing infection, entirely without clinical symptoms. Two attempts to infect with the first strain failed, although the culture was left in contact for 8 and 9 nights respectively.