Development of free-living amoebae on agar in the presence of polyols. Study of 10 strains

Perrine, D.; Barbier, D.; Georges, P.

Comptes Rendus des Seances de la Societe de Biologie 172(4): 633-639


Reference strains of 10 species of free-living amoebae (Naegleria gruberi, Hartmannella vermiformis, Vahlkampfia avara and Acanthamoeba spp.) were cultured on agar plates. Addition of various concentrations of polyols (ethylene glycol, propylene glycol or glycerol) to the agar inhibited excystment and growth to a greater or lesser extent. After 3 weeks on the modified agar most cysts were able to excyst when transferred to a standard medium.