Population study of the sclerotia of Macrophomina phaseolina in cotton fields
Sheikh, A.H.; Ghaffar, A.
Pakistan Journal of Botany 7(1): 13-17
A technique was developed for the isolation and enumeration of Macrophomina phaseolina from soil. After wet sieving through 120 mesh screen the soil residue retained on 300 mesh screen was suspended in 0.5% Calcium hypochlorite solution and distributed on PDA Streptomycin, Demosan and rose bengal. M. phaseolina colonies were detected within 5 days giving containing Penicillin, 94% recovery. Naturally infested soils averaged 0-29 sclerotial propagules per gram of soil. More sclerotia were present in the surface soil than at 6" depths. A substantially higher sclerotial population detected in certain root rot infested patches as compared to healthy fields. In difference in the population of sclerotia between the healthy and root rot infested portions of cotton general no significant was fields was observed.