An improved HPLC method for estimation of phyllanthin and hypophyllanthin in Phyllanthus amarus
Murali, B.; Amit, A.; Anand, M.S.; Dinesh, T.K.; Samiulla, D.S.
Journal of Natural Remedies 1(1): 55-59
An Isocratic, reversed phase HPLC procedure has been adopted using a mixture of pH 2.8 phosphate buffer and acetonitrile as mobile phase, CN column as stationary phase and UV detector to develop an improved HPLC method for estimation of phyllanthin and hyphophyllanthin in P. amaranthus (collected from Ponnamali, Chennai, India in February 1999).