Neurosecretion and its physiological correlation in hypopituitary hereditary dwarf mice


Stouter, V.P.

Dissertation Abstracts 1959: 429-430

1959


ZOOLOGY
429
lacks
the
characteristic
structure
of
the
metraterm
common
to
monorchiids.
It
is
of
further
interest
that
the
family
Microphallidae
is
represented
by
2
new
species,
Carneophallus
lactophrysi
and
Megalophallus
diodontis.
Both
genera
have
been
re-
ported
from
birds
or
mammals
only.
This
is
the
first
record
of
microphallids
occurring
in
marine
fishes.
It
is
necessary
to
redefine
the
family
Waretrematidae
to
include
2
new
genera
Neomegasolena,
which
lacks
a
hermaphroditic
sac,
and
Allomegasolena,
which
is
very
similar
to
the
genus
Megasolena,
but
differs
in
several
respects.
Both
of
the
new
genera
have
two
testes,
and
the
cuticle
is
spinose.
Neomegasolena
is
represented
by
a
single
species,
N.
chaetodipteri,
and
Allomegasolena
by
two
species,
A.
spinosa
and
A.
attenuata.
The
families
Microscaphiidae
and
Gorgoderidae
are
each
represented
by
a
single
species,
and
3
are
reported
for
each
of
the
families,
Cryptogonimidae,
Megaperidae
and
Opistholebetidae.
Five
previously
known
species
be-
longing
to
the
Acanthocolpidae
are
described,
4
in
the
genus
Stephanostomum
and
Manteria
brachydera,
previ-
ously
known
only
from
Pacific
waters.
The
families
Opecoelidae
and
Lepocreadiidae
are
especially
well
represented,
the
Opecoelidae
by
22
spe-
cies
and
the
Lepocreadiidae
by
21
species.
A
new
opeco-
elid
genus
Pinguitrema
is
erected
to
include
P.
lobata,
which
shows
characteristics
of
both
the
genus
Pycnadena
and
members
of
the
family
Opistholebetidae.
The
genus
Neohelicometra
is
erected
to
contain
N.
cablei
which
has
separate
ani,
but
otherwise
resembles
the
genera
Heli-
cometra
and
Helicometrina
in
having
eggs
with
unipolar
filaments.
Of
three
species
of
Hamacreadium,
two
are
described
as
new:
H.
lintoni
and
H.
longisaccum.
Among
the
Lepocreadiidae,
a
new
genus
Neoapocread-
ium
has
been
erected
to
contain
N.
angustum,
N.
coili,
and
N.
bravoi,
all
described
as
species
of
Apocreadium
by
Sogandares-Bernal
(in
press).
Four
new
species
are
described
in
the
genus
Neolepidapedon
and
are
reported
for
the
first
time
from
Atlantic
waters.
The
genus
Lepi-
dapedon
is
represented
by
a
single
new
species
L.
holo-
centri.
Microfilm
$2.85;
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$9.80.
217
pages.
STUDIES
ON
MARINE
FISH
TREMATODES
FROM
THE
GULF
OF
PANAMA
AND
BIMINI,
BRITISH
WEST
INDIES
(L.
C.
Card
No.
Mic
59-1792)
Franklin
Sogandares-Bernal,
Ph.D.
The
University
of
Nebraska,
1958
Adviser:
Professor
H.
W.
Manter
During
the
summers
of
1956
and
1957
the
author
col-
lected
trematode
parasites
from
fishes
in
the
Gulf
of
Panama
(1956)
and
at
Bimini,
B.W.I.
(1957).
In
Panama,
234
specimens
of
115
species
of
fishes
were
examined;
at
Bimini,
250
specimens
of
109
species;
a
total
of
480
individuals
of
222
species.
Eighty-six
species
of
digenetic
trematodes
were
col-
lected
and
studied.
Thirty-one
of
these
were
from
Pan-
ama,
sixty
from
Bimini.
Five
species
occurred
in
both
regions.
Twenty-nine
new
host
records
are
noted.
Two
new
genera
and
seventeen
new
species
are
named.
These
are
distributed
in
eight
families.
The
names
of
ten
species
and
one
genus
are
consid-
ered
synonyms
of
forms
previously
named.
Observations
on
the
ecology
of
these
trematodes
in-
cluded
notation
of
the
exact
location
of
the
parasites
in
the
digestive
tract,
and,
in
some
cases,
measurement
of
the
pH
(alkalinity
or
acidity)
of
these
locations.
The
pH
was
found
to
vary
considerably
even
in
the
same
region
of
different
individuals,
depending
chiefly
on
recency
of
feeding.
The
Digenea
of
Bimini
are
very
similar
to
those
known
from
Tortugas,
Florida
where
they
have
been
extensively
studied.
However,
the
known
resemblance
of
those
of
Tor-
tugas
to
those
of
the
American
Pacific
is
increased
by
5
species
found
in
both
oceans,
making
an
impressive
total
of
41
species.
Microfilm
$2.80;
Xerox
$9.60.
214
pages.
NEUROSECRETION
AND
ITS
PHYSIOLOGICAL
CORRELATION
IN
HYPOPITUITARY
HEREDITARY
DWARF
MICE
(L.
C.
Card
No.
Mic
59-1988)
Vincent
P.
Stouter,
Ph.D.
The
University
of
Buffalo,
1959
By
use
of
the
Gomori
chrom-alum
hematoxylin
stain-
ing
technique,
neurosecretory
deposits
in
the
hypothalarno-
neurohypophyseal
system
of
dwarf
mice
are
shown
to
be
more
prominent
than
in
normal
young
mice
with
body
di-
mensions
similar
to
dwarfs
(size-controls)
and
in
normal
adult
mice
of
ages
comparable
to
dwarfs
(age-controls).
The
neurosecretory
content
of
the
supraoptic
and
para-
ventricular
nuclei
of
dwarfs
is
considerably
more
dense
than
in
normal
size-controls
and
age-controls.
The
infundibular
process
of
severely
saline-dehydrated
or
water-deprived
dwarfs
sacrificed
on
the
point
of
death
shows
a
residuum
of
neurosecretory
material
which
is
not
observed
in
similarly
treated
size-controls.
The
expla-
nation
of
this
finding
is
uncertain.
It
is
suggested
that
the
more
prominent
neurosecretory
deposits
in
the
hypo-
thalamo-neurohypophyseal
systems
of
dwarfs
and
the
infundibular
neurosecretory
residuum
in
severely
dehy-
drated
dwarfs
is
the
resultant
of
a
less
efficient
release
mechanism
at
the
neurohypophyseal
nerve
terminals.
The
administration
of
mildly
hydrating
or
severely
hydrating
water
loads
to
dwarfs
by
stomach
tube
does
not
increase
the
amounts
of
hypothalamo-neurohypophyseal
neurosecretory
material.
Single
doses
of
adrenal
cortical
extract
(ACE)
or
multiple
doses,
given
in
the
period
of
2
-
3
hours,
do
not
appreciably
alter
the
neurosecretory
content
of
the
infundibular
process.
Pitressin
adminis-
tered
in
a
similar
fashion
is
also
shown
to
have
little
effect
in
the
alteration
of
the
infundibular
neurosecretory
content.
The
daily
water
requirements
and
urine
outputs
of
dwarfs
are
not
materially
different
from
size-controls.
Dehydration
by
water-deprivation
and
2%
saline
adminis-
tration
is
tolerated
by
dwarfs
and
normals
to
approxi-
mately
the
same
extent,
i.
e.,
7
and
10
days,
respectively.
Dwarfs
are
more
susceptible
to
water
intoxication
than
are
normal
size-controls.
The
items
following
each
abstract
are:
the
number
of
manuscript
pages
in
the
dissertation
and
its
cost
on
microfilm.
Enlargements
5-1/2
x
8-1/2
inches,
4
cents
per
page.
No
postage
is
charged
if
check
or
money
order
accompanies
order.
430
ZOOLOGY
Water
intoxication
can
be
prevented
in
both
dwarfs
and
size-controls
by
the
simultaneous
administration
of
ACE
along
with
successive
water
doses.
Susceptibility
to
water
intoxication
is
increased
proportionally
in
dwarfs
and
size-controls
by
the
administration
of
pitressin.
Microfilm
$2.00;
Xerox
$5.20.
101
pages.
STUDIES
ON
THE
DEVELOPMENT
AND
APPLICATION
OF
A
MODIFIED
HEMAGGLUTINATION
PROCEDURE
(L.
C.
Card
No.
Mic
59-1831)
Helen
R.
Strausser,
Ph.D.
Rutgers
University,
1959
Major
Professor:
Dr.
Ralph
J.
DeFalco
This
thesis
is
concerned
with
the
development
of
a
modified
hemagglutination
technique
based
on
the
Boyden
method
of
treating
sheep
erythrocytes
with
tannic
acid
thus
rendering
them
capable
of
adsorbing
protein
mole-
cules
from
solution.
Erythrocytes
which
have
been
coated
in
this
manner
are
agglutinated
by
high
dilutions
of
anti-
sera
directed
against
the
protein
molecules.
To
facilitate
the
performance
of
the
Boyden
procedure,
and
to
overcome
the
occasional
lack
of
reproducibility
due
to
variations
in
different
samples
of
erythrocytes,
as
well
as
to
improve
the
stability
of
the
erythrocytes
for
test
and
storage
pur-
poses,
a
method
by
which
the
cells
are
preserved
with
formalin
has
been
used.
Formalin
preserved
erythrocytes
of
various
species
have
been
stable
and
serologically
re-
active
for
periods
up
to
twenty-one
months
at
the
time
of
this
writing.
Storage
of
the
entire
antigen
coated
pre-
served
cell
complex
has
been
accomplished
by
the
use
of
additional
preservatives
or
freezing
of
the
coated
pre-
served
cells.
The
modified
test
has
been
applied
to
the
determination
of
the
specificity
of
anti-beef
and
anti-sheep
sera
when
tested
with
homologous
and
heterologous
antigens
and
sim-
ilar
determinations
with
anti-beef
albumin
and
anti-beef
globulin
sera
have
been
performed.
In
addition,
the
mod-
ified
hemagglutination
test
has
been
used
to
detect
human
antibodies
to
tuberculin
and
egg
albumin
and
rabbit
anti-
bodies
to
chorionic
gonadotrophin.
Inhibition
of
the
he-
magglutination
reaction
has
been
used
to
determine
the
specificity
of
the
antisera
as
well
as
to
detect
the
presence
of
the
antigen,
e.g.
detection
of
the
presence
of
chorionic
gonadotrophin
in
pregnancy
urine.
Advantages
and
disadvantages
of
the
use
of
preserved
cells
in
hemagglutination
technique
as
compared
with
freshly
washed
erythrocytes
are
discussed.
It
is
suggested
that
results
be
read
by
means
of
a
sedimentation
method.
The
anti-globulin
test
has
been
found
to
be
useful
in
improving
the
sensitivity
of
the
preserved
cell
hemagglu-
tination
reaction
providing
precautions
are
taken
to
pre-
vent
the
adsorption
of
non-specific
proteins
on
the
tanned
erythrocytes.
Microfilm
$2.00;
Xerox
$4.80.
91
pages.
The
items
following
each
abstract
are:
the
number
of
manuscript
pages
in
the
dissertation
and
its
cost
on
microfilm.
Enlargements
5-1/2
x
8-2/2
inches,
4
cents
per
page.
No
postage
is
charged
if
check
or
money
order
accompanies
order.