Rearing of Tabanus nigrovittatus Macquart (Diptera: Tabanidae) from egg to adult
Sofield, R.K.; Hansens, E.J.
Journal of the New York Entomological Society 88(1): 75
Host-seeking female salt marsh greenheads, Tabanus nigrovittatus, were collected from box traps on a salt marsh near Cedarville, NJ. Flies were allowed to blood feed on human forearm or restrained guinea pig. Ten egg masses were oviposited by these flies. The egg masses turned grey-brown with a chalky covering several hours after oviposition. The egg masses were loosely cemented together. Each egg mass was removed from the cage and placed on moist filter paper in a petri dish until hatching occurred. Seven masses hatched after 5 days yielding 508 larvae. Larvae were reared at 27 C in individual 8 dram vials with wet filter paper. Every 2-5 days the filter paper was changed and fresh food (house fly maggot or earth worm) provided. Larval mortality was highest in the first 40 days. Only 12.5% of the larvae survived this period. Three to five months after hatching, half the larvae were subjected to 5 C for 40 days and the remainder for 80 days. The rearing temperature was then returned to 27 C. Pupation of 26 larvae from four egg masses occurred 6-9 months after hatching. A single larva pupated 60 days after hatching. The pupal stage lasted 7-12 days. Of the 14 females reared, 7 oviposited a mass of infertile eggs, 3 took a blood meal after oviposition, and one fly oviposited a second mass of infertile eggs.