Gradient thin layer chromatography of coumarins and furocoumarins
Glowniak, K.; Matysik, G.; Bieganowska, M.; Soczewinski, E.
Chromatographia 22(7-12): 307-310
Extracts of four umbelliferous plants containing coumarins and furocoumarins were chromatographed in sandwich chambers with a glass distributor. Best separations were obtained for stepwise gradient elution in the concentration range of 2.5 to 15% ethyl acetate in chloroform. Seven eluent fractions with increasing ethyl acetate concentrations were introduced directly under the distributor of the sandwich chamber.