The Difil Test kit for detection of canine heartworm microfilariae
Tilley, L.P.; Wilkins, R.J.
Veterinary Medicine, Small Animal Clinician 69(3): 288-294
The Difil Test Kit appears to be a reliable working application of the filtration technique for the detection and differentiation of circulating microfilariae in the blood. The technical improvements and modification in lysing solution, staining technique and use of a clear transparent filter membrane have minimized residual cellular debris and eliminated many of the problems inherent in the original method. Because the filters effectively trap all microfilariae in the blood sample, quantitation of microfilariae recovered is possible, providing a known blood volume is used. In addition to its reliability, the filter technique is very rapid; no centrifugation is involved and little time is required to scan the slide. This method should allow practitioners to screen blood samples for circulating microfilariae quickly and accurately.